影响内皮细胞凋亡的因素及其机制探讨
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内皮细胞；凋亡；基因表达,刘韧肖南田昆仑刁有芳陈惠孙
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刘韧 肖南 田昆仑 刁有芳 陈惠孙
摘 要：目的：观察亚砷酸钠(Ars)，内毒素(LPS)、肿瘤坏死因子(TNF)、烧伤血清、创伤血清对内皮细胞凋亡的影响及其机制。方法：体外培养内皮细胞ECV-304，与上述物质孵育24小时后检测凋亡水平及ICAM-1.bcl-2、p53表达的变化。结果：Ars可使内皮细胞发生剂量依赖的凋亡增加(与对照相比P<0.01)但是LPS、TNF、烧伤血清和创伤血清对内皮细胞的作用有限，细胞凋亡率在15%以下。除10%烧伤血清外，所有处理因素都使内皮细胞I-CAM-1表达降低。体外诱导内皮细胞凋亡与bal-2表达降低、p53表达增多有关。结论：Ars、LPS、TNF烧伤血清和创伤血清bc1-2表达、上调P53表达来影响内皮细胞凋亡，并且抑制内皮细胞功能。
关键词：内皮细胞；凋亡；基因表达
分类号：Q25 文献标识码：A
文章编号：1001-0726(2001)02-0065-05
Substances That Induced Endothelial Cell Apoptosis and the Mechanisms Involved
Liu Ren(Research institute of surgery, Daping Hospital, The 3rd Military Medical Unversity, Chongqing, 400042)
Xiao Nan(Research institute of surgery, Daping Hospital, The 3rd Military Medical Unversity, Chongqing, 400042)
Tian Kun-lun(Research institute of surgery, Daping Hospital, The 3rd Military Medical Unversity, Chongqing, 400042)
Abstract：Objective: To investigate the endothelial cell apoptosis inducing effect of substances, including sodium arsenite (Ars), lipopolysaccharide (LPS), tumor necrosis factor (TNF), burned serum and traumatic serum and the mechanisms involved. Methods: By comparing the changes of apoptosis rates and the expressions of ICAM-1, bc1-2, p53 after endothelial cells ECV-304 were incubated with these apoptosis inducing substances.Results: Ars increased the apoptosis rate of endothelial cells and this effect was dose-dependent (compared with control, P〈0.01).LPS,TNF,burned serum and traumatic serum had only limited effect on endothelial cell apoptosis,the apoptosis rate was below 15%.Except for 10%burned serum,all these substances decredsed the expression of endothelial cell ICAM-1,in vitro en-dothelial cell apoptosis inducing effect were correlated to the decrease in the expression of bc1-2and the increase in the expression of p53 and IGAM-1.Conclusion:Ars, LPS,TNF,burned serum and traumatic serum influenced endothelial cell apoptosis through down-regulation of bc1-2 and up-regulation of p53 and inhibited their functions.
Keywords：Endothelial cell； Apoptosis； Gene expression
作者简介：刘韧 (1966- ),女 (汉族 )，河南固始人，四川大学毕业，助理研究员 . 肖南 (1963- )，男 (汉族 )，四川南充人，第三军医大学毕业，副研究员 . 田昆仑 (1964- )，男 (汉族 ) ......
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