【摘要】 目的 观察阿霉素(Doxorubicin，DXR)诱导HSP72对大鼠肝脏长时间冷保存的保护作用。方法 实验组SD大鼠按1mg/kg DXR给药，对照组给予生理盐水，48h后用4℃林格液和UW液行肝脏原位冷灌注，肝脏UW液4℃保存24、36、48h，检测保存液谷草转氨酶(ALT)、谷丙转氨酶(AST)、乳酸脱氢酶(LDH)、透明质酸(HA)平，并行肝组织HE染色、HSP72免疫组化和电镜观察。结果 大鼠肝脏UW液保存243648h，DXR组保存液AST、ALT、HA浓度显著低于对照组(P<0.05)，而LDH浓度两组差异无显著性(P>0.05);组织病理及电镜结果显示，DXR组肝组织形态基本正常，肝细胞及肝窦内皮细胞表达HSP72，对照组肝组织损伤严重，无HSP72表达。结论 阿霉素预处理可使肝脏冷保存时间延长并保持其活性;HSP72的表达可以保护肝脏抵御长时间冷保存造成的损伤。

    关键词 阿霉素 HSP72 大鼠肝脏 低温保存

    protective effect of HSP72induced by pretreatment of doxorubicin for long term preservation of rat liver

    Chen Hao，Deng Xiaxing，Yang Weiping，et al.

    Reijin Hospital of Shanghai2nd Medical University，Shanghai200025.

    【Abstract】 Objective To observe the protective effect of HSP72induced by pretreatment of Doxorubicin(DXR)for long-term cold preservation of the rat liver.Methods The Sprague(Dawley rats were administered intravenously by DXR at a dose of1mg/kg body weight in DXR group and by saline in control group.After48hours，the rat liver was perfused by using cold Linger’s and University of Wisconsin(UW)solutions and then was preserved in UW solution at4℃for24 ......