【摘要】 目的 黄芩苷(Baicalin，Bai)是中药黄芩的主要有效成分之一，属于黄酮类化合物，有抑菌、抗炎、抗变态反应、抗氧化反应等药理作用，本文探讨黄芩苷对脂多糖所致星形胶质细胞(astrocytes，AC)损伤的保护作用及其机理。方法 体外培养新生SD大鼠原代AC，MTT分析法检测黄芩苷对正常组与脂多糖干预的星形胶质细胞细胞活力，逆转录多聚酶链式反应(RT-PCR)检测星形胶质细胞iNOS mRNA的表达。结果 5μmol，10μmol，20μmol浓度的黄芩苷能恢复LPS导致损伤的星形胶质细胞的活力(F=3.697，P=0.01)。脂多糖干预后iNOS mRNA的表达明显增多，黄芩苷可以抑制脂多糖所致的AC iNOS mRNA的表达(P<0.05，n=5)，有量效关系。以20μmol的浓度黄芩苷对iNOS mRNA的表达抑制最明显。结论 黄芩苷能够抑制脂多糖导致AC的损伤，可能与其抑制iNOS mRNA的表达有关。

    Zhang Shaofang，Liu Jiebo，Yuan Shaoqun

    Depantment of Pediatrics，Luohu Hospital，Shenzhen518001.

    【Abstract】 Objective To explore the protective effect and mechanism of Baicalin on injury induced by LPS in astrocytes of rat.Methods Rat astrocytes were cultured in vitro，and cell viability was assayed by MTT about the effect of Baicalin on normal astrocytes and the astrocytes treated byLPSTheconcentration of NO 2- /NO 3- in superˉnatantwas evaluatedbyspectrocolorimeter.NO 2- /NO 3- are assayed by spectrophotometer.RT-PCR was used to measure the expression of iNOS mRNA.Results There were no influences on the cells viability of astrocytes cultured with different concentrations(5μmol ......