【摘要】 目的 观察经重组人干细胞因子(rhSCF)+重组人粒细胞集落刺激因子(rhG-CSF)动员后外周血干细胞(PBSC)自身输注对环磷酰胺化疗猴造血功能恢复的影响。方法 15只成年猕猴分成正常对照、化疗对照和rhSCF50μKD+rhG-CSF3组。rhSCF+rhG-CSF组于单独注射rhSCF3天后再联用rhG-CSF每天1次，rhSCF的给药剂量为50μg/(kg·d)(μKD)，rhG-CSF为10μKD，给药时间分别为8天和5天;化疗对照组给予等体积的生理盐水。化疗前一天收集PBSC，化疗后24h自身回输，观察其造血恢复情况。结果 rhSCF+rhG-CSF可使正常猴外周血白细胞和CFU-GM数升至256%和370%，动员的PBSC自身回输后化疗猴外周血白细胞、血小板数及骨髓造血祖细胞集落数明显高于对照组。结论 rhSCF+rhG-CSF对正常猴PBSC有明显动员作用，动员的PBSC可明显促进环磷酰胺化疗猴造血功能恢复。

    关键词 重组人粒细胞集落刺激因子 重组人干细胞因子 化疗 外周血干细胞

    The effect of autologous infusion of rhSCF+rhG-CSF-mobilized peripheral blood stem cells on hematopoiesis of rhesus monkeys treated with cyclophosphamide

    Hao Jing，Guo Dehuang，Xiong Guoling，et al

    Institute of Radiation Medicine，Academy of Military Medical Sciences，Beijing100850.

    【Abstract】 Objective To evaluate the effect of autologous infusion of rhSCF+rhG-CSF-mobilized periphˉeral blood stem cells(PBSC)on the hematopoietic recovery of cyclophosphamide treated rhesus monkeys.Methods 15healthy adult rhesus monkeys were divided into normal control ......