【摘要】 目的 探讨塞来昔布对大肠癌细胞侵袭能力和转移能力的影响，以及对肿瘤血管生成的影响。方法 用Matrigel体外侵袭实验检测塞来昔布对LoVo细胞体外侵袭能力的影响;建立Lovo细胞裸鼠肝转移模型，检测塞来昔布对LoVo细胞肝转移能力的影响;用CD34免疫组化染色方法检测肝转移瘤的微血管密度(MVD)，了解塞来昔布对肿瘤血管生成的影响。结果 塞来昔布能显著降低LoVo细胞的体外侵袭能力。塞来昔布能显著减少裸鼠LoVo细胞肝转移模型的肝转移数量，对照组98.83±10.42，用药组63.4±5.59(P<0.01)。用药组的微血管密度均明显低于对照组(P<0.01)。结论 塞来昔布能明显降低LoVo细胞的体外侵袭能力和在裸鼠体内的肝转移能力，并能抑制转移瘤的血管生成。

    关键词 塞来昔布 大肠癌 侵袭 转移 血管生成

    The influences of celecoxib on the invasive and metastatic abilities of colorectal cancer cells

    Zhao Leiguang，Wan Yuanlian，Zhu Jing.

    Department of General Surgery，the First Hospital of Beijing University，Beijing100034.

    【Abstract】 Objective To study the influences of celecoxib on the invasive and metastatic abilities of colorecˉtal cancer cells，and the influence on tumor angiogenesis.Methods Matrigel invasion assay was used to observe in vitro the influence of celecoxib on the invasive ability of LoVo cells.The liver metastasis model was established in nude mice to illustrate in vivo the effect of celecoxib on the metastatic ability of LoVo cells.Microvessel density of the metastatic nodules was analyzed by immunohistochemistry in CD34-stained tumor sections to observe the influence of celecoxib on tumor angiogenesis.Results In vitro invasive ability of LoVo cells was significantly inhibited by treating with celecoxib.Nude mice of treated group developed fewer liver metastatic nodules than those of control group ......