【摘要】 目的 观察同型半胱氨酸对正常妊娠晚期孕妇血小板L-arg/NO系统的影响及意义。方法 选择正常妊娠晚期孕妇12例，采用放射性核素 3 H-L-arg标记法，分别测定正常妊娠晚期孕妇(对照组)及经同型半胱氨酸孵育过(Hcy组)的血小板内L-arg转运、NOS的活性，比色法测定血小板内NO含量。结果 经同型半胱氨酸孵育过的血小板L-arg转运为(3.70±0.72)pmol/108pt·min -1 ，正常妊娠晚期孕妇血小板L-arg转运为(5.82±1.35)pmol/108pt·min -1 (P<0.01);对照组在Ach刺激前后NOS活性、NO产量均较相应的Hcy组为高(P<0.05或P<0.01);在Ach刺激后Hcy组NOS活性、NO分别增加了7.03%及31.58%，而对照组分别增加了39.38%及46.15%，较Hcy组明显增多。结论 同型半胱氨酸影响血小板L-arg的转运，使NOS的活性降低，血小板内NO水平降低，同型半胱氨酸损伤血小板L-arg/NOS/NO系统。

    关键词 同型半胱氨酸 妊娠晚期 血小板 L-arg转运 NOS活性 NO

    Homocysteine on L-arginine/nitric oxide pathway of platelets in third-trimester pregnancy

    Wang Jurong，Yan Hua，Wang Zhiwen，et al.

    Department of Obstetrics and Gynecology，Handan Central Hospital，Handan056001.

    【Abstract】 Objective To study the mechanism of damage in platelets function caused by homocysteine in third-trimester pregnant women，by observing the changes of the L-arg/NO pathway of platelet.Methods Twelve third-trimester pregnantwomen were enrolled for this study.Half of every plasma sample was incubated byhomocysteine.The velocity of L-arg transport ......