【摘要】 目的 探讨血管内皮生长因子C(VEGF-C)及其受体Flt-4在人非小细胞肺癌(NSCLC)组织中的表达及其与淋巴结转移等临床病理特征的关系。方法 采用免疫组化S-P法检测60例NSCLC原发灶组织、10例癌旁组织、32例伴有肺癌转移的淋巴结组织中VEGF-C、Flt-4的表达，并将结果与肺癌的临床病理特征进行分析。结果 VEGF-C和Flt-4在转移的淋巴结癌细胞中、NSCLC和癌旁组织中的表达各组间差异有显著性(均P<0.05);且VEGF-C与Flt-4在肺癌细胞中的表达呈正相关(P<0.05);VEGF-C表达与肺癌TNM分期正相关(P<0.05);VEGF-C和Flt-4表达与淋巴结转移正相关(P<0.0，P<0.01)，与肿瘤组织类型、病理分级无关;NSCLC中Flt-4阳性微脉管数在淋巴结转移组明显高于无淋巴结转移组，差异有非常显著性(P<0.001)。结论 在NSCLC组织中VEGF-C水平上调，是由肺癌细胞分泌，并通过自分泌方式作用于细胞膜上的Flt-4受体，与非小细胞肺癌的发生有一定关系;VEGF-C与NSCLC中淋巴管的生成及淋巴结转移密切相关。

    关键词 肺肿瘤 血管内皮生长因子 血管内皮生长因子受体-3 淋巴转移

    Expression and its correlation with lymph nodes metastasis of vascular endothelial growth factor-C(VEGF-C)and vascular endothelial growth factor receptor-3(Flt-4)in non-small cell lung cancer

    Zheng Xiaohua，Zhao Wei，Zhang Peiyi，et al.

    202Hospital of PLA，Shenyang110003.

    【Abstract】 Objective To study the gene expression of vascular endothelial growth factor C(VEGF-C)and vascular endothelial growth factor receptor-3(Flt-4)in non-small cell lung cancer and their relation with pathologˉic features.Methods S-P immunohistochemical staining was used to detect the expression of VEGF-C and its reˉceptor Flt-4in10neighboring noncancerous tissue and60NSCLC cases of non-small cell lung cancer and32lymph nodes cases with lymph nodes metastasis of non-small cell lung cancer.Results The difference of positive expression rate of VEGF-C and Flt-4in NSCLC and paracancer tissues and lymphatic node metastasis was signifiˉcant(P<0.05)respectively.A positive correlation was found between VEGF-C and Flt-4in lung cancer cell(P<0.05).The expression of VEGF-C of NSCLC correlated with the TNM stages(P<0.05).The expression of VEGF-C and Flt-4of NSCLC was closely related to lymph node metastasis(P<0.01P<0.01).VEGF-C ......