Orexin-A mRNA表达的脏器分布及在急性炎症反应中的变化
颜光涛 林季 廖杰【摘要】 目的: 研究Orexin-A mRNA表达在体内主要脏器的分布及在急性炎症损伤后的变化，探讨Orexin-A在急性炎症反应中的作用。方法: 取正常雄性SD大鼠和肠缺血-再灌注(IR)损伤模型大鼠的下丘脑、胃、肺、肾、附睾脂肪垫、睾丸、肝、脾、十二指肠等主要脏器，采用逆转录-聚合酶链反应(RT-PCR)检测Orexin-A mRNA在各主要脏器的分布情况，同时检测IR损伤后下丘脑内Orexin-A mRNA的表达变化。结果: 所取各脏器均有Orexin-A mRNA表达，尤其是在胃、肺、肾的表达丰度较高。在IR损伤的急性刺激作用后，下丘脑Orexin-A mRNA表达水平出现波动式的下降趋势。结论: Orexin-A mRNA广泛分布于机体主要脏器，对急性炎症刺激具有时间依赖性的应答效应，其表达水平变化可以作为一种新型的急性炎症反应监测指标。
【关键词】 Orexin-A mRNA； 缺血-再灌注损伤,肠； 逆转录-聚合酶链反应
Distribution of Orexin-A mRNA expression in different organs and its variation in acute inflammation
YAN Guang-tao, LIN Ji, LIAO Jie.
Research Laboratory of Biochemistry, General Hospital of PLA, Beijing 100853, China
【Abstract】 OBJECTIVE:：To explore the distribution of Orexin-A mRNA expression in different organs, and its variation in acute inflammation, and to evaluate the role of Orexin-A in acute inflammatory responses. METHODS:：Hypothalamus, adipose tissue, lung, stomach, duodenum, liver, spleen and testis of male Sprague-Dawley rats (including normal and those of intestinal ischemia/reperfusion injury model) were harvested, and reverse transcription-polymerase chain reaction(RT-PCR) was used to assess Orexin-A mRNA expression in different tissues of normal rats, and that in the hypothalamus of ischemia/reperfusion injured rats. RESULTS: All tissues expressed Orexin-A mRNA, and it was especially high in the stomach, lung and kidney. After the acute stimulus of ischemia/reperfusion injury, Orexin-A mRNA expression showed a trend of fluctuant declination in the hypothalamus. CONCLUSION:：Orexin-A mRNA is widely expressed in the major organs, and it has a time-dependent response to acute inflammatory stimuli. The results suggest that its expression variation may be used as a novel marker for acute inflammation. ......
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