【摘要】 目的 研究细菌生理状态(不同生长时期)对中药抑菌活性的影响。方法 采用营养琼脂稀释法测定几种中药对不同生理状态(指数生长期、稳定期、衰亡期)的大肠杆菌的最低抑菌浓度(MIC)。结果 观察到指数生长期的大肠杆菌对多种中药的MIC较高，约为衰亡期细菌MIC值的2～8倍。但新霉素对大肠杆菌的MIC值几乎不受细菌生理状态的影响。结论 细菌的生理状态显著影响中药的抑菌活性。提示在测定中药的抑菌活性和中药使用过程中，应该注意细菌生理状态的影响作用，以便得到可靠的实验结果，取得更好的疗效。

    关键词 中药 细菌 MIC 细菌生理状态

    The influence of bacterial physiological state on the antibacterial effect of Chinese herbal medicinesJin Jianling，Hua Guoqiang，Chao Bo，et al.

    State Key Laboratory of Microbial Technology，Shandong University，Jinan250100.

    【Abstract】 Objective To study the effect of bacterial physiological stateson the inhibitive activity of Chinese herbal medicines.Methods E.coli cells in different physiological state were used to test their minimal inhibtion conˉcentration(MIC)to Chinese herbal medicines by nutrient-agar dilution method.Results Exponential phase E.coli cells had higher MIC to herbal medicines(2～8times higher)than that of death phase cells;but both exponential phase and death phaseE.coli cells had smilar MIC to neomycin.Conclusion Bacterial physiological states noteˉworthily influence the antibacterial effect of Chinese herbal medicines. ......