FOP-FGFR1 tyrosine kinase, the product of a t(6;8) translocation induces a fatal myeloproliferative disease in mice
From the Institut National de la Santé et de la Recherche Médicale (INSERM) U 119, IFR 57, Marseille, France; Université de la Méditerranée, Marseille, France; Department of Hematology Institut Paoli Calmettes, Marseille, France; Department of BioPathology, Institut Paoli Calmettes, Marseille, France; and INSERM EMI-0116, IFR 57, Marseille, France.w'(y\1, 百拇医药
Constitutive activation of aberrant fibroblast growth factor receptor 1 (FGFR1) kinase as a consequence of gene fusion such as FOP-FGFR1 associated with t(6; 8)(q27;p11-12) translocation, is the hallmark of an atypical aggressive stem cell myeloproliferative disorder (MPD) in humans. In this study, we show that expression of FOP-FGFR1 in primary bone marrow cells induced by retroviral transduction generates a MPD in mice. Constitutive FOP-FGFR1 kinase activity was both essential and sufficient to cause a chronic myeloproliferative syndrome in the murine bone marrow transplantation model. In contrast to the human disorder, lymphoproliferation and progression to acute phase were not observed. Lymphoid symptoms, however, appeared when onset of the disease was delayed as the result of mutation of FOP-FGFR1 at tyrosine 511, the phospholipase C {gamma} (PLC{gamma} ) binding site.(Géraldine Guasch Bénédicte Delaval Christine Arnoulet Min-Jue Xie Luc Xerri Danielle Sainty Danie)
Constitutive activation of aberrant fibroblast growth factor receptor 1 (FGFR1) kinase as a consequence of gene fusion such as FOP-FGFR1 associated with t(6; 8)(q27;p11-12) translocation, is the hallmark of an atypical aggressive stem cell myeloproliferative disorder (MPD) in humans. In this study, we show that expression of FOP-FGFR1 in primary bone marrow cells induced by retroviral transduction generates a MPD in mice. Constitutive FOP-FGFR1 kinase activity was both essential and sufficient to cause a chronic myeloproliferative syndrome in the murine bone marrow transplantation model. In contrast to the human disorder, lymphoproliferation and progression to acute phase were not observed. Lymphoid symptoms, however, appeared when onset of the disease was delayed as the result of mutation of FOP-FGFR1 at tyrosine 511, the phospholipase C {gamma} (PLC{gamma} ) binding site.(Géraldine Guasch Bénédicte Delaval Christine Arnoulet Min-Jue Xie Luc Xerri Danielle Sainty Danie)