【摘要】 目的 探讨CIK细胞(cytokine-induced killer CIK)体外对肿瘤细胞的细胞毒活性。方法 从健康人外周血分离淋巴细胞，经过IFN γ 、CD3McAb、IL-2诱导并培养，获得大量的CIK细胞。FACS检测CIK细胞的表型;以CFSE标记靶细胞来区分效应细胞，再以PI染色，用FACS检测靶细胞的死亡率。结果 CIK细胞高度表达CD3约88.03%CD3CD56表达35.05%，CD16表达不增加。CIK细胞对肿瘤细胞K562、HL-60、Hela、7721、A375均表现出杀伤活性，其中对K562、HL-60、Hela三种细胞的杀伤作用较强;而对7721、A375的作用不明显。结论 三种细胞因子体外诱导的单个核细胞具有强大的增殖力和杀伤活性。

    关键词 CIK细胞 细胞毒性 流式细胞术

    Yang Guangmin，Duan Xuimei，Yang Mi，et al.

    Central laboratory of Jilin Provincial Hospital，Changchun130021.

    【Abstract】 Objective To probe into the cytotoxicity of CIK cells against tumor cells in vitro.Methods Lymphocytes cells were isolated freshly from peripheral blood of health donors by Ficoll-Hypaque density centrifugaˉtion，and the cells obtstained were induced by IFNγ，IL-2and CD3mAb.Phenotypes of CIK cells were analysed by FACS.Target cells were differentiated from effect cells by CFSE dying.The mortality of Target cells were determined by FACS.Results The phenotypes of CD3were expressed highly(88.03%)、CD3CD56(35.05%).The expression of CD16were not increased.CIK cells possess the cytotoxicity against tumor cells of K562、HL-60、Hela、7721and A375.Conclusion IFN γ 、CD3McAb、IL-2can induced strong peripheral lymphocytes to produced CIK cells which exert highly efficient cytotoxic effects on tumor cells. ......