Zhao Haidong，Xiao Xuren，Zhang Haibin，et al.

    The466th Hospital of the Peoples Liberation Army of China，Beijing100089.

    【Abstract】 Objective To find whether the donor-specific tem cell can induce immunolgical tolerance and esˉtablish a simple、effective and practical protocal of inducing donor-specific tolerance.Methods A mouse model of skin transplantation was established.HBC in the donor BMwere collected by StemSep TM (StemSep TM is a negative selection sysˉtem in which the unwanted cells are immunomagnetically labeled and bound to a magnetic column.The desired cells colˉlected

    in the collumn flowthrough，have not had antibody bound to their surface).BALB/C mice were treated with3.5Gy TBI，then were fed50mg/kg CsA.Immediately，treated mice received fully mismatched B6BMC or B6HSC.Within the same day after completing the conditioning regimen，skin transplantation from B6to BALB/C was performed.Survival time of the grafts was recorded.Mixed lymphocyte reaction(MLR)and delayed type hypersensitivity(DTH)assay were perˉformed on day30to examine the tolerance status.Results Time of the grafts survival was significantly prolonged.BALB/C recipients were specifically tolerant to host antigens in MLR and DTH assays ......