【摘要】 目的 观察川芎嗪对实验性急性出血坏死性胰腺炎肺损伤大鼠的治疗作用，并对其作用机理进行探讨。方法 取Wistar大鼠108只，随机分为假手术组、模型组、川芎嗪组3组。经胆胰管逆行注射5%牛磺胆酸钠(1ml/kg体重，0.1ml/min)复制急性出血坏死性胰腺炎模型，在造模后30min经尾静脉注射川芎嗪(80mg/kg)，术后3h、6h、12h取血，测定血清淀粉酶、脂肪酶、PLA 2 、TNFα、IL-1、IL-6;记录腹水量，测定腹水淀粉酶、脂肪酶、PLA 2 ;取左肺一部分匀浆，测定MPO、PLA 2 、TNFα、IL-1、IL-6，一部分经10%甲醛固定作病理组织学检查，取右肺进行肺泡灌洗，分离收集并纯化肺泡巨噬细胞，提取核蛋白后采用化学发光ELISA法检测NF-κB(P65)活性;留取胰腺组织进行组织病理学检查;观察各组24h死亡率。结果 川芎嗪能明显降低AHNP大鼠24h死亡率;减轻肺组织及胰腺的病理性损伤;降低血中、肺组织中及腹水中PLA 2 活性;减少腹水生成量;降低血中、肺组织中TNFα、IL-1、IL-6水平;显著降低肺泡灌洗液巨噬细胞NF-κB活性。结论 川芎嗪能有效抑制急性胰腺炎时增高的PLA 2 活性，抑制NF-κB的活化，下调多种细胞因子的表达，减轻肺组织损伤。

    on acute hemorrhagic necrotizing pancreatitis-associated lung injury

    Liu Hongbin，Cui Naiqiang，Li Donghua，et al.

    Tianjin Institute of Acute Abdominal Diseases，Tianjin300100.

    【Abstract】 Objective To explore the therapeutic mechanisms of tetramethylpyrazine on acute lung injury inˉduced by acute hemorrhagic necrotizing pancreatitis in rats.Methods One hundred-eight Wistar rats were randomly divided into sham operation group，AHNP group，tetramethylpyrazine group.AHNP model was induced by intraductul administration of5%sodium taurocholate.Tetramethylpyrazine(80mg/kg)was administrated by caudal vein injection30minutes after model induction.Additional animals were used to observe the24hour-mortality.Animals were killed on various hours after model induction(3h ......