【摘要】 目的 探讨胆汁细菌学培养的检出率及耐药状况，指导临床合理使用抗生素。方法 对145例胆道疾病患者胆汁进行细菌培养，用美国Microscan Walkway40全自动细菌分析仪进行鉴定。结果 从145例胆汁标本中分离出病原菌92株，培养阳性率达63.4%，其中革兰阳性球菌50株，占54.4%，革兰阴性杆菌38株，占41.3%，真菌4株，占4.35%，药敏结果显示革兰阴性杆菌对亚胺培南敏感率高，革兰阳性球菌对万古霉素敏感率高，对青霉素类，一、二、三代头孢菌素明显耐药。结论 胆道感染的主要致病菌为大肠埃希菌、肺炎克雷伯菌、屎肠球菌、粪肠球菌和葡萄球菌属，常出现多重耐药。

    Li Fang

    Department of Laboratory Medicine，Beijing，Chaoyang Hospital Affiliated to the Capital University of Medical Science，Beijing，100020，China

    【Abstract】 Objective To investigate the positive rate of the biliary bacterial cultures and drug resistance for the guidance of rational use of antibiotics.Methods Biliary specimens from145inpatients with biliary disease were cultured and the balterial identification were appraised with the American Microscan Walkway40.Results A total of92strains were separated from145biliary specimens ......