【摘要】 目的 探讨淫羊藿水提液对体外培养的乳鼠颅骨成骨细胞活性的影响。方法 用胰蛋白酶和胶原酶消化乳鼠颅骨获得成骨细胞，取第三代培养细胞加入不同浓度的淫羊藿水提液，观察成骨细胞增殖情况、 3 H-脯氨酸( 3 H-Pro)摄入量和碱性磷酸酶活性的变化。结果 各淫羊藿实验组成骨细胞增殖均明显增加，碱性磷酸酶(ALP)活性和 3 H-Pro摄入也有不同程度的增加。结论 淫羊藿具有增强成骨细胞活性的作用。

    Wang Xianyuan，Xu Zhiqin，Zhang Yuehong，et al.

    Institute of Hygiene and Environmental Medicine，Military Medical Science Academy，Tianjin300050.

    【Abstract】 Objective To study the effect of epimedium on activity of rat osteoblast in vitro.Methods Osˉteoblast，derived from skull digested by trypsin and collagenase from natal rat，were added epimidium to study multipliˉcation，intake of 3 H-proline and activity of ALP.Results Multiplication，ALA activity and intake of 3 H-proline were increased in different extend in every experimental group.Conclusion Epimidium could boost up activity of osˉteoblast.

    Key words epimidium osteoblast 3 H-TdR 3 H-Pro ALP

    骨质疏松是一种全身性的骨代谢疾病 ......