【摘要】 目的 观察生血宁治疗失血性贫血的药效及对铁代谢平衡调节的基因机制。方法 将大鼠随机分成正常对照组、失血模型对照组、琥珀酸亚铁对照组100mg/(kg·d)、生血宁治疗组100mg/(kg·d)，每组10只。除正常对照组外，其它3组每天尾端放血2ml，连续6天，使造成失血性贫血模型。第6天开始灌药或水。第6、16、24天取血测血常规，第24天取十二指肠标本用RT-PCR法测铁代谢相关基因DMT-1、IREG1、Hephaestin的mRNA表达量。结果 生血宁能提高红细胞计数、血红蛋白、红细胞比积、红细胞平均血红蛋白浓度、缩小红细胞体积分布宽度。生血宁在基因水平能改善铁代谢，降低失血性贫血大鼠铁代谢相关基因DMT-1、IREG1、ephaestin的表达，疗效与琥珀酸亚铁相似。结论 生血宁治疗失血性贫血效果明显;在基因水平能改善铁代谢，降低DMT-1、IREG1、Hephaestin。

    关键词 叶绿素盐 失血性贫血 铁代谢 阳离子转运蛋白 基因表达调节

    An experimental study on Sheng Xuening treating hemorrhagic anemia and imp

    roving iron metabolism

    Ke Youfu，Wei Kemin，Zheng Junxian，et al.

    Zhejiang Institute of Traditional Chinese Medicine，Hangzhou310012.

    【Abstract】 Objective To observe the efficacy of Sheng Xuening on treating hemorrhagic anemia and its gene mechanism of regulating iron metabolism.Methods 40rats were randomly divided into normal control group，hemorˉrhagic anemia model control group，ferrous succinate control group100mg/(kg·d)and Sheng Xuening treatment group100mg/(kg·d).The other three groups except normal control group got blood bled2milliliters a day for6days conˉsecutively to make anemia model.Drug or water was given everyday by oral gavage respectively beginning at the sixth day.Blood routine examination was measured at the sixth ......