【摘要】 目的 研究豆楮方对人肝癌细胞株HepG 2 的生长增殖抑制作用，从分子生物学角度探讨其抗癌作用机制。方法 文献法制备豆楮方含药血清，用MTT法观察药物作用24h、48h后细胞的增殖情况。结果 豆楮方含药血清在体外对人肝癌细胞株HepG 2 有显著的抑制作用，作用24h的最高抑瘤率为91.48%，作用48h的最高抑瘤率为90.31%，抑瘤效果优于5-FU。结论 豆楮方具有抗肝癌作用。

    关键词 豆楮方 人肝癌细胞株HepG 2 实验研究

    The Experimental Research on the Inhibiting action of Dou-Chu Formula on the Proliferation of HepG 2 cell line in vitro

    Peng Haiyan，Zhang Xiaochun，Zhang Yonghong，et al.

    The First Clinical Medical College，Traditional Chinese Medicine University of Nanjing，Nanjing210029.

    【Abstract】 Objective To observe the inhibiting action of Dou-Chu formula on the proliferation of HepG 2 cell line in vitro，so as to discuss its anticancer mechanisms.Methods Obtain pharmaceutic serum of the rabbit after feeding itwith Dou-Chu formula for5days.HepG 2 cell line was treated with the pharmaceutic serum at different conˉcentrations for24h and48h respectively.The inhibiting rate of cells was observed byMTT.Results The proliferation of HepG 2 cells was obviously inhibited after being treated with Dou-Chu formula.The best effect for24h was91.48%and that of48h was90.3%.Conclusion Dou-Chu formula has anticancer function. ......