【摘要】 目的 茶多酚是从绿茶中提取的水溶性多酚类物质，皮肤吸收较困难。本文主要探讨茶多酚固体脂质纳米粒乳液的体外透皮性能。方法 采用微乳液法制备茶多酚固体脂质纳米粒乳液，利用离体兔皮和自制透皮装置对茶多酚固体脂质纳米粒乳液进行体外透皮实验。在不同时间收集接受液样本，用紫外分光光度法测定其吸光度，根据茶多酚标准曲线计算接受液的浓度，根据累积透过量公式计算其累积透过量。将累积透过量对时间进行线性拟合，得回归方程，方程的斜率即为渗透系数。结果 茶多酚标准曲线的回归方程为y=0.0315x-0.011，r=0.9972。不同时间点的接受液吸光度逐渐增加，平均值从0.5h的0.048增加到24h的0.272;对应的浓度和累积透过量也随着时间的延长而增加。累积透过量对时间的回归方程为y=19.121x+86.577，r=0.9988，渗透系数为19.121。结论 茶多酚固体脂质纳米粒乳液具有较好的皮肤透过性，透过量随时间延长而增加，累积透过量亦呈时间依赖性;且该种制剂透皮过程平稳。

    关键词 茶多酚 固体脂质纳米粒乳液 透皮实验

    Experimental study of transdermal test of solid lipid nanopraticles of tea polyphenols

    Lin Xiangfei，Luo Dan，Min Wei，et al.

    Department of Dermotology and Venereology，the First Affiliated Hospital of Nanjing Medical University，Nanjing210029.

    【Abstract】 Objective Tea polyphenols are the active ingredients in green tea and possess antioxidant，phoˉtoprotective and anti-carcinogenic properties.Tea polyphenols are hydrophilic and they are difficult to be absorbed by the skin.In our study，we focus on the transdermal capacity of tea polyphenols solid lipid nanopraticles(TPSLN)through the rabbit skin.Methods TPSLN was prepared by microemulsion technique and the concentration was meaˉsured by HPLC method.The ......