【摘要】 目的 检测胃癌患者外周血CD3 + T细胞分泌细胞因子的水平变化，分析Th1和Th2细胞的细胞因子免疫活动，为肿瘤的免疫治疗提供实验依据。方法 首先用刺激物刺激细胞，增加细胞内细胞因子的表达。然后加入荧光标记的特异性抗细胞因子单克隆抗体，特异性抗原抗体结合，最后应用流式细胞仪分析特异性细胞因子表达水平。同时用酶联免疫吸附法(ELISA)检测相应的细胞因子。结果 胃癌患者Th1型细胞因子干扰素-γ(IFN-γ)、白细胞介素-2(IL-2)、白细胞介素-12(IL-12)表达水平较正常对照组显著降低，Th2型细胞因子白细胞介素-4(IL-4)和白细胞介素-10(IL-10)表达水平较正常对照组升高，差异有统计学意义。肿瘤坏死因子-α(TNF-α)表达水平较正常对照组升高，差异有显著性。结论 胃癌患者体内Th2型细胞因子模式占优势状态，这可能是肿瘤细胞发生免疫逃逸，从而导致肿瘤的发生或者转移的原因之一。

    关键词 胃癌 细胞因子 Th1/Th2 流式细胞术

    Clinical signficance of Th1/Th2cytokines in the peripheral blood

    lymphocytes of patients with gastric cancer

    Wu Ping，Chen Jie，He Huijuan，et al.

    The Central Laboratory，Affiliated Hospital of Guangdong Medical College，Zhanjiang524001.

    【Abstract】 Objective To detect the level of Th1and Th2type cytokines of peripheral blood CD3 + T lymˉphocytes in the patients with gastric cancer.In order to find out the laboratory evidence of tumor immunotherapy.Methods The peripheral blood lymphocytes were stimulated by stimulator in order to enhance expression of cyˉtokines.Specific fluorochrome-conjugated monoclonal antibody combinedwith antigen.Cytokines were detected by flow cytometry and ELISA.Results The level of Th1type cytokines such as interferon-γ(IFN-γ) ......