【摘要】 目的 为评估锝标记抗D-二聚体单抗在兔早期股动脉血栓定位诊断中的应用。方法 将 99m Tc标记的抗D-二聚体单抗4D30注入兔体内，进行放射免疫显像。分别观察血栓部位和对侧相应部位的放射性分布，计算放射性比值;显像完毕后取血栓及周围肌肉组织，称重后测定单位质量的放射性计数及比值;取心、肝、脾、肺、肾及膀胱组织，观察病理学改变。结果 股动脉血栓形成6h内，注入显像剂后30min，血栓开始显像。血栓/血液和血栓/周围肌肉单位质量放射性比值分别为2.08±0.33，3.68±0.74。各器官均未见明显病理学改变。结论 提示 99m Tc-4D30可以用于实验性早期动脉血栓的定位诊断。

    关键词 锝 单克隆抗体 D-二聚体 血栓

    99m Tc labeled anti-D-dimer monoclonal antibody for the diagnosis of early femoral arterial thrombus of rabbit

    Liu Yu，Dong Xiaoli，Wang Jue，et al.

    Department of Pathology，Capital University of Medical Science，Beijing100054.

    【Abstract】 Objective To evaluate the uslefulness of technetium( 99m Tc)labelled monoclonal anti-D-dimer antibody(4D30)for target diagnosis of early femoral arterial thrombus of rabbit.Methods Radioimaging and quantitative radioactive analysis were performed by SPECT after 99m Tc-4D30was injected.Results Thrombus-to-blood and thrombus-to-muscle ratios were counted with gamma counter.The morphologic changes in heart，liver，spleen，lung，kidney and bladder were observed under light microscope.The femoral arterial thrombi showed at30min after injection(6h after the thrombi formed).Thrombus-to-blood and thrombus-to-muscle ratios were2.08±0.33and3.68±0.74.No pathologic change in all organs was observed under lightmicroscope.Conclusion It is concluded that the 99m Tc-laˉbelled anti-D-dimer monoclonal antibody(4D30)can be regarded as one of potential target agent in diagnosis of early femoral arterial thrombus. ......