【摘要】 目的 探讨在6P21.3的PPPIRl0基因鼻咽癌发生的作用。方法 采用肿瘤相关基因确定候选基因，用黄金分割优选法，采取根据类似疾病(肿瘤)的相关基因来确定候选基因，用PCR扩增引物片段，预变性，根据条件设计DNA片段分析系统(DHPLC)有关参数进行自行运行检测，从而找出鼻咽癌易感基因的位点。27例原发性鼻咽癌和正常人对照外周血标本189例，进行了检测。结果 在6P21.3TNFa/至微卫星标记D6P1640之间基因，用PCR方法扩增分布于候选基因中10个片段含有编码区SNPs序列，DHPLC检出511个杂合子，证实该片段杂合子为一个新的位点，可能为鼻咽癌易感基因。结论 通过基因定位所限定的候选基因内部或附近的SNPs进行相连性研究，成为预测复杂性、多基因疾病性质、原因及易感性的有效方法。

    关键词 鼻咽肿瘤 易感基因 单核苷酸多态性

    Study on SNPs and susceptibility gene of NPC

    Yao Liangquan，Li Xin，Guo Yuehua，et al.

    Department of Surgery，Shenzhen TCM Hospital Affiliated to Guangzhou Uniuersity of TCM，Shenzhen518033.

    【Abstract】 Objective To investigate the correlation between PPPIR10gene at6P21.3and nasopharyngeal carcinoma(NPC).Methods The candidate genes were determined from cancer-related genes with golden section optimum selection method.The genes were amplified by PCR and the products were analyzed by high-porformance liquid chromatography(DHPLC)to find the site of susceptibility gene of NPC.The blood samples from27patients with NPC and189healthy subjects were analyzed.Results At6P21.3from D6P1640 ......