【摘要】 目的 模拟临床检测酸性磷酸酶(ACP)的试验条件，探讨其对ACP活性测定的影响。方法 采用不同的试管收集静脉血标本，在不同时间、不同条件下分离血清并检测ACP总活性(ACPT)和耐酒石酸酸性磷酸酶(TrACP)活性，观察其变化规律。结果 低温离心后血清置室温1h内测定ACPT和TrACP活性与其真实值较为一致;血清置室温2.5～3.5h酶活性下降约11%。离心后未及时分离血清或全血置室温2.5h再分离血清可使测定结果偏高，后者可使酶活性升高达20%。促凝胶可使血清ACPT显著升高，但对TrACP影响不显著。柠檬酸钠抗凝和氟化钠—草酸钾抗凝血浆ACPT均比普通真空管明显降低，尤以后者对ACP活性的抑制更显著。结论 不同试验条件可使ACPT与TrACP的测定结果产生明显差异，做好分析前的质量控制对ACP活性测定非常重要。

    关键词 酸性磷酸酶 试验条件 酶活性

    Effects of changes of different experimental conditions on

    determination results of acid phosphatase

    Jiang Xianyong，Liu Qingwu，Huang Rong

    The Affiliated Hospital of Xiangnan University，Chenzhou423000.

    【Abstract】 Objective To explore the effects of changes of different experimental conditions on determination results of acid phosphatase(ACP).Methods Different tubes were used to collect the samples of venous blood，and blood serum was separated under different conditions and at different times，and ACPT and the activity of TrACP were determined.Results ACPT and the activity of TrACP were determined after the centrifugation of serum in low temˉperature and the serum in room temperature for an hour ......