【摘要】 目的 克隆Nogo-c的cDNA序列，并构建其真核表达载体，为进一步研究其抑制神经生长机制奠定基础。 方法 根据基因GenBakn中Nogo-c基因的碱基序列，利用RT-PCR的方法从鼠脑组织中扩增编码Nogo-c基因，将目的片段与pMD18T载体连接，利用亚克隆的方法将Nogo-c的cDNA片段克隆到pcDNA3.1载体中，并进行序列测定。 结果 DNA测序证实该片段序列与文献报道完全一致。 结论 成功构建出pcDNA3.1-Nogo-c真核表达载体。

    关键词 克隆 Nogo-c 真核表达载体

    Cloning and sequencing of Nogo-c and construction of its eucaryotic expression vector

    Chen Changjie，Zhang Yao

    Department of Molecular Biology and Biochemistry of Bengbu Medical College，Bengbu233003.

    【Abstract】 Objective To construct recombinant eukaryotic expression vector containing Nogo-c gene se-quence and to pave the way for understanding how the gene inhibits neuron growth.Methods The Nogo-c cDNA was obtained from mouse brain tissue by RT-PCR.The target gene was cloned into pMD18T vector ......