【摘要】 目的 探讨一种简便、敏感、特异的肝吸虫检验方法。 方法 以成虫粗蛋白为抗原，用ELISA方法检测肝吸虫病患者血清中的肝吸虫抗体。肝吸虫病患者均为大便检出虫卵。设健康人及其他疾病患者作对照。 结果 肝吸虫病患者血清31例，本法阳性30例;其他疾病患者血清102例，本法阳性10例;健康人血清65例，本法阳性5例。本法敏感性为96.8%，特异性为93.1%;诊断准确度为93.1%。 结论 本法敏感性高，操作比较简便，可作为肝吸虫病的初诊及普查筛选方法。

    关键词 肝吸虫 酶联免疫吸附实验

    Application of ELISA in the diagnosis of hepatic distomiasis

    Li Suifen，Tan Yizhou，Chen Weilie

    Guangzhou Eighth People's Hospital，Guangzhou510060.

    【Abstract】 Objective To discuss a convenient，sensitive and specific test technique to detect hepatic disto-miasis.Methods The antibody of hepatic distomiasis in the serum of clonorchiasis sinensis patients was detected with antigen of distomiasis imago coarse protein by ELISA.Worm eggs of distomiasis were detected in the stool of every clonorchiasis sinensis patients.Set healthy people and patients with other diseases as normal controls.Results The antibody of hepatic distomiasis was detected in30out of31serums of clonorchiasis sinensis patients by ELISA.10/102of patients with other diseases were detected the antibody in serum.And5/65of healthy people's antibody of hepatic distomiasis in serumwere positive.The sensitivity of ELISA to detect the hepatic distomiasis was96.8% ......