【摘要】 目的 探讨人参、甘草体外诱导人外周血单个核细胞(PBMC)分泌细胞因子机制。 方法 我们选择生晒参、红参、甘草三种中药的提取液加入PBMC培养液中，孵育5d后，采用ELISA方法测定上清液中的细胞因子水平。 结果 生晒参、红参、甘草能诱导PBMC分泌细胞因子和SIL-2R，其中药组TNF-α、SIL-2R、IL-2、IFN-γ含量明显高于细胞对照组，特别是生晒参诱生PBMC高分泌IFN-γ尤为突出。 结论 生晒参、红参、甘草对体外诱导PBMC分泌TNF-α、IL-2、IFN-γ细胞因子和SIL-2R。

    【关键词】 生晒参 红参 甘草 PBMC 细胞因子

    Studies on the role of induced cytokines，SIL-2by ginseng and glycyrrhiza in PBMC

    Zhang Jianping，Xu Keyi，Gao Yanjing，et al.

    Institute of Infectious Disease，Beijing Ditan Hospital，Beijing100011.

    【Abstract】 Objective In order to elucidate the role of ginseng and glycyrrhiza in cellular immunity regulation and study the mechanism of induced cytokines by the Chinese medicines in vitro.Methods PBMC were induced by water-solubility extract respectively from radix ginseng ......