【摘要】 目的 研究还原型谷胱甘肽对乳鼠心肌细胞缺氧/复氧(A/R)损伤的影响。 方法 建立心肌细胞A/R损伤模型，随机分为5组:A组，正常对照组;B组，单纯缺氧/复氧(A/R低氧2h，复氧1h)组;C、D、E组为还原型谷胱甘肽处理组，加入还原型谷胱甘肽(GSH)分别使其终浓度为40、80、160mg/L，后A/R。于复氧后测定各组培养液中乳酸脱氢酶(LDH)变化及细胞内丙二醛(MDA)含量和细胞存活率。 结果 与正常组相比，单纯低氧/复氧组LDH漏出量、MDA水平显著升高(P<0.01)，细胞存活率显著降低(P<0.01)。与缺氧/复氧组比较，各谷胱甘肽处理组上述变化明显减轻(P<0.05)。 结论 还原型谷胱甘肽对乳鼠心肌细胞A/R损伤具有保护作用，并具有浓度依赖性。

    关键词 还原型谷胱甘肽 心肌细胞 缺氧 复氧

    The effects of reduced glutathione sodium on anoxia/reoxygenation injuries of neonatal rat cardiomyocytes

    Wang Jin，Wang Yibiao，Yang Haiying，et al.

    The Second Affiliated Hospital of Shandong University of TCM，Jinan250001.

    【Abstract】 Objective To study the effects of reduced glutathione sodium(GSH)on anoxia/reoxygenation in-juries of neonatal rat cardiomyocytes.Methods A/R model of myocardial cells of neonatal rats was established.The cells of neonatal rats were randomly divided into five groups:control group(A):without any treatment;A/R group(B):2-hour anoxia followed by1-hour reoxygenation;GSH conditioned group(C):adding GSH into culture medium till the final concentration of40mg/L then A/R;GSH conditioned group(D):adding GSH into culture medium till the final concentration of80mg/L then A/R;GSH conditioned group(E):adding GSH into culture medium till the final concen-tration of160mg/L then A/R.The activity of lactate dehydrogenase(LDH) ......