【摘要】 目的 研究免疫抑制剂对小鼠腹腔巨噬细胞(Macrophage，M)的损伤作用机制。 方法 以小鼠腹腔巨噬细胞为对象，采用小鼠尾静脉注射地塞米松0.15mg/只，环磷酰胺2.0mg/只，小鼠腹腔巨噬细胞培养，检测小鼠腹腔巨噬细胞吞噬功能及腹腔灌洗液中NO 2- /NO 3- 浓度并用形态学方法观察比较免疫抑制剂对小鼠腹腔巨噬细胞的损伤特点。 结果 地塞米松和环磷酰胺均能抑制巨噬细胞功能，与对照组比，细胞吞噬功能均显著下降(P<0.01)，DEX组腹腔灌洗液中NO 2- /NO 3- 浓度显著升高，Cy组不升高。VitC为自由基链反应阻断剂，阻断过氧化链式反应可减轻损伤，可使DEX组细胞吞噬功能获得部分恢复。 结论 推测环磷酰胺对腹腔巨噬细胞损伤是以直接损伤染色体而DEX以氧化损伤为主。

    【关键词】 地塞米松 环磷酰胺 巨噬细胞

    The effect of dexamethasone and cyclphosphamide on peritoneal macrophage of mice

    Yuan Wendan，Gao Feng，Chen Jinrong，et al.

    Department of Pathophysiology，Shandong BinZhou Medical College，Shandong256603.

    【Abstract】 Objective We investigated demage mechanism of peritoneal macrophage(M)induced by injec-tion of dexamethasone and cyclophosphamide in mice.Methods The immune function by determining the phagocytic function and structure of the macrophagocytes of the abdominal cavities of mice in vitro.The phagocytosis of peritoneal macrophages was evaluated by chicken erythrocytes method;Observed the concentratuon of NO -2 /NO -3 in the peri-toneal lavage fluid.Results Dexamethasone and cyclophosphamide induced the apoptosis of peritoneal macrophage and concomitant decrease of phagocytosis(vs control group ......