培养心肌细胞牵张刺激装置的建立及应用
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席雨涛;马爱群;白晓君;吴格如
牵张刺激|心肌细胞|血管紧张素Ⅱ,关键词:
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席雨涛;马爱群;白晓君;吴格如 西安交通大学第一医院心内科 陕西西安710061 第四军医大学学报 2003 3
关键词:牵张刺激;心肌细胞;血管紧张素Ⅱ
AIM: To set up a set of stretch equipment in vitro to study the mechanism of cadiomyocyte hypertrophic response to stretch. METHODS: The gel stretch device was made from silica gel and organic glass, etc. Ang Ⅱ was measured by radioimmunoassay. The protein synthesis was tested by MTT. These two parameters were used to assess the effect of this device. RESULTS: Under 20% stretch force for 12h or 24h, the protein synthesis was obviously increased and the auto secretion of Ang II also rose. But the numb...
关键词:牵张刺激;心肌细胞;血管紧张素Ⅱ
AIM: To set up a set of stretch equipment in vitro to study the mechanism of cadiomyocyte hypertrophic response to stretch. METHODS: The gel stretch device was made from silica gel and organic glass, etc. Ang Ⅱ was measured by radioimmunoassay. The protein synthesis was tested by MTT. These two parameters were used to assess the effect of this device. RESULTS: Under 20% stretch force for 12h or 24h, the protein synthesis was obviously increased and the auto secretion of Ang II also rose. But the numb...
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