大鼠阴茎组织NOS活性及海绵体平滑肌细胞NO含量的增龄变化
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孔祥东；胡曼菁；姚茂银；宋萍
勃起功能障碍|一氧化氮|一氧化氮合酶|细胞培养|平滑肌细胞,关键词：
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孔祥东；胡曼菁；姚茂银；宋萍 东南大学中大医院中医内科；东南大学中医院泌尿外科；东南大学临床医学院中心实验室 江苏南京210009 第四军医大学学报 2003 1
关键词：勃起功能障碍；一氧化氮；一氧化氮合酶；细胞培养；平滑肌细胞
We investigated the effect of aging on the mechanism of erctile dysfunction (ED) in rats and the posibility of NO release from rat penile cavernous smooth muscle cells (RPCSMC). NOS activity in penile tissue (2 , 8 and 16 months rats) and NO contents in RPCSMC were detected. Immunocytochemistry was used to deterimne NOS activity. The results showed that NOS activity (23±5,11±2 ,4±1) kU?g -1 and NO contents (138±9, 76±4, 22±1) mmol?L -1 decreased significantly with aging ( P <0.01) .
关键词：勃起功能障碍；一氧化氮；一氧化氮合酶；细胞培养；平滑肌细胞
We investigated the effect of aging on the mechanism of erctile dysfunction (ED) in rats and the posibility of NO release from rat penile cavernous smooth muscle cells (RPCSMC). NOS activity in penile tissue (2 , 8 and 16 months rats) and NO contents in RPCSMC were detected. Immunocytochemistry was used to deterimne NOS activity. The results showed that NOS activity (23±5,11±2 ,4±1) kU?g -1 and NO contents (138±9, 76±4, 22±1) mmol?L -1 decreased significantly with aging ( P <0.01) .
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