牛平 王耀山 吕永利 何祥 牛平 王耀山 何祥(沈阳军区总医院神经内科，辽宁 沈阳 110015)；吕永利(中国医科大学基础医学院解剖教研室，辽宁 沈阳 110001) 中风与神经疾病杂志 2000 0 17 4


    关键词：脑源性神经营养因子；帕金森病；6-羟基多巴胺；酪氨酸羟化酶 期刊 zfysjjbzz 0 209-211 论著与经验总结 fur -->


    

摘 要： 目的 观察脑源性神经营养因子(Brainderived-neurotrophic factor,BDNF)对帕金森病(Parkinson disease,PD)大鼠模型黑质多巴胺能神经元的影响。方法 将6-羟基多巴胺用立体定向法注入大鼠一侧中脑黑质制作PD大鼠模型，并在注入6-羟基多巴胺之间每天向预伤侧壳核区定位注入BDNF，采用酪氨酸羟化酶免疫组化方法及透射电镜等观察BDNF对黑质多巴胺能神经元的显微超微结构影响。结果 BDNF能改善6-羟基多巴胺造成的PD大鼠黑质多巴胺能神经元数目减少及超微结构影响。结论 BDNF可减轻6-羟基多巴胺对黑质多巴胺能神经元的毒性作用。

    中图分类号：R742.5 文献标识码：A 文章编号：1003-2754(2000)04-0209-03

Effectsof Brain derived neurotrophic factor (BDNF) on substantial nigra dopaminergic neurons inParkinson disease rats

NIUPing WANG Yao-shan LU Yong-li et al.

    ( Department ofNeurology,The General Hospital of Shenyang Command,Shenyang 110015 China )

Abstract ： Objective To study the effects of brainderived neurotrophic factor on substantial nigra dopaminergic neurons in Parkinson diseaserats. Methods Ratswere lesioned by unilateral injection of 6-OHDA into the substantial nigra in order tocreat animal models of Parkinson disease. Before injection of 6-OHDA,we infused BDNF intothe putaman for 2 weeks,then counted the numbers of TH positive neurons and observed themicroscopic,ultrastructure changes by tyrosine hydroxylase immunohistochemistry andtransmission electron microscope. Results In groups treated by BDNF,the numbers of dopaminergicneuron significantly increased in comprision to control groups. The microscopic andultrastructure also changed slightly. Conclusion BDNF can reduce and prevented dopamine neurons againstneurotoxin of 6-OHDA in vivo.

     Key words: Brainderived neurotrophic factor ； Parkinson disease ； 6-OHDA ； Tyrosinehydroxylase ▲

帕金森病(Parkinsondisease,PD)是慢性、进行性神经系统变性病 ......

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