魏敏 周莉玲 李锐 广州中医药大学，广州 510405 中药新药与临床药理 2000 0 11 1


    关键词：丁公藤注射液；东莨菪内酯；药代动力学；血浆 期刊 zyxyylcyl 0 11-13 血清药理学研究 fur -->


    

摘 要： 用 PR-HPLC 法测定丁公藤注射液有效成分总东莨菪内酯在小鼠血浆中的浓度。色谱条件：SpheriSorb_C₁₈ 不锈钢柱，流动相为甲醇-水-冰醋酸(38∶62∶0.19)，检测波长 345 nm。血浆样品经酸水解后用氯仿萃取，50℃水浴，氮气吹干，甲醇超声溶解后进样。本法具有快速、简便、灵敏、准确等特点，已经用于总东莨菪内酯血药浓度测定及药代动力学研究，结果满意。

    分类号： R969.1 文献标识码： A

    文章编号： 1003-9783(2000)01-0011-03

Determination of Plasma Concentration of Total Scopoletin by RP-HPLC

WEI Min, ZHOU Li-ing, LI Rui

( Guangzhou University of TCM, Guangzhou 510405)

Abstract： The plasma concentration of total scopoletin in Dinggongteng Injection was determined by RP_HPLC. The chromatographic conditions were as follows: stainless column Spherisorb C₁₈ ; mobile phase:methanol_water_glacial acetic acid (38∶ 12∶ 0.19)； detection wave length 345nm. The plasma sample was extracted by chloroform after acid hydrolysis, dried by nitrogen on a water bath at 50℃ , and dissolved with methanol. This method is rapid simple, sensitive and accurate, and has been used in plasma concentration determination and pharmacokinetics of total scopoletin.

     ......