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关键词：唾液；蛋白水解酶；乳牙龋；性别
摘要 目的: 分析测定儿童唾液中的蛋白水解酶活性,研究其与性别和龋敏感性的关系。方法: 采用酶-底物反应法和考马斯亮蓝蛋白含量测定法，分别测定不同性别、不同龋敏感性儿童唾液中亮氨酸氨肽酶、甘氨酰脯氨酸二肽酶和胰酶样蛋白酶的活性及总蛋白含量。结果: ①在儿童唾液中仅能测得甘氨酰脯氨酸二肽酶和胰酶样蛋白酶，且其活性明显低于牙菌班中的酶活性(P<0.05);②唾液中蛋白水解酶活性与儿童性别和龋齿数无关(P>0.05)。结论: 儿童唾液中蛋白水解酶的生物学作用尚需进一步研究。
The Study of theActivities of Salivary Proteolytic Enzymes in Children
Qi Xue, Zhang Jingyi,Li Shaomin,et al
College of Stomatology, West China University of Medical Sciences
Abstract
Objective: The activities of proteolytic enzymes in saliva were assayed todetermine whether they were related to caries sensitivity and gender in the children. Methods: Fluorometric and photometric methods were applied. Three enzymes included leucine aminopeptidase, dipeptidyl peptidase Ⅳ and trypsin-like proteinase. Results: ①Onlydipeptidyl peptidase Ⅳ and trypsin-like proteinasewere demonstrated in saliva, they were obviously lower than those in dental plaque(P<0.05); ②The activities of proteolytic enzymesof male and female children as well as caries-active and caries-free children showed nodifference. Conclusion: Proteolytic enzymes play little role in cariesformation of deciduous tooth.
Key words: saliva proteolytic enzyme deciduous caries gender
唾液中的蛋白水解酶多来自细菌 ......
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