关键词：再灌注损伤；肌，骨骼；缺血预处理；天冬氨酸转氨酶；肌酸激酶；兔
【摘要】 目的 观察缺血预处理对骨骼肌缺血再灌注损伤的保护作用。方法 选择24只健康兔，随机等分为实验组和对照组。实验组先进行缺血预处理，再持续阻断后肢血流4h；对照组直接阻断后肢血流4 h，制作骨骼肌缺血再灌注损伤模型。测定再灌注期血清中肌酸磷酸激酶(CPK)和天门冬氨酸氨基转移酶(AST)，镜下观察骨骼肌结构变化。结果 实验组血清中CPK和AST的含量均明显低于对照组(P<0.05)。实验组骨骼肌线粒体空泡变性和肌原纤维溶解程度明显轻于对照组。结论 缺血预处理能增强骨骼肌对缺血的耐受性，减轻骨骼肌缺血再灌注损伤程度。
【中图分类号】 R685.4; R364.2; R345
Ischemic preconditioning reduces ischemia and reperfusion injury of skeletal muscle
Tang Shouying, Wang Xiaojun
(Department of Orthopaedics, The 251st Hospital of PLA, Zhangjiakou, Hebei 075000)
【Abstract】 Objective To observe the effect of protecting skeletal muscle fromischemia and reperfusion injury with ischemic preconditioning. Methods 24 healthy rabbits wereselected and randomly divided into either experiment or control group equally.The model ofischemia and reperfusion injury was made by stopping blood flows of rabbits hind limbs for 4 hours, theexperiment preconditioned and the control undergoing nothing.The contents of creatinephosphate kinase (CPK) and aspartate aminotransferase (AST) in the serum were measuredrespectively during reperfusion. Structural changes of the skeletal muscle were observedwith light and electron microscope. Results The contents of CPK and AST in the experimental group werelower than that in the control (P<0.05). Compared with the control, mitochondrialvacuolar degeneration and dissolution of myofibril in the experimental group weresignificantly reduced. Conclusion IPC enhances the continuous ischemic tolerance of skeletalmuscle and reduces its ischemia and reperfusion injury.
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