曾庆彪 王振国 甘润良 彭燕玲 曾庆彪(衡阳医学院第一附属医院病理科 湖南省衡阳市421001)；王振国(衡阳医学院第一附属医院病理科 湖南省衡阳市421001)；甘润良(衡阳医学院第一附属医院病理科 湖南省衡阳市421001)；彭燕玲(衡阳医学院第一附属医院病理科 湖南省衡阳市421001) 中国肿瘤临床 2000 0 27 2


    关键词：妊娠滋养细胞疾病；c-Ha-ras基因突变；p21蛋白 期刊 zgzllc 0 104??107 论著 fur -->


    

摘要 目的：探讨c-Ha-ras基因突变及p21蛋白表达与妊娠滋养细胞疾病(GTD)发生发展的关系，寻找具有特异性的可预测PTD的诊断标记。方法：采用PCR-SSCP法及SP免疫组化法，对90例GTD组织中c-Ha-ras基因第1外显子点突变及其产物p21表达进行检测，以正常足月新鲜胎盘30例为对照。结果：所有正常胎盘组织ras突变及p21表达均为阴性。ras基因突变在恶性GTD中为36.7%，CM将来发展成为PTD的为43.3%，均高于CM无PTD发展的16.7%。恶性GTD,Ⅰ、Ⅱ、Ⅲ级组织分化者点突变率分别为15.4%、40%、71.4%，各级间差异有显著性

C - Ha - ras Gene Mutation and p21 Expression in Gestational Trophoblastic Disease

Zeng Qingbiao Wang Zhenguo Gan Runliang et al
(Department of Pathology, First Teaching Hospital of Hengyang Medical College, Hunan Province )

Abstract Objective : To study the relationship between the mutation of C - Ha - ras gene and gestational trophoblastic disease (GTD) and to identify diagnostic marker for predicting GTD. Methods : The mutation in exon 1 of C - Ha - ras gene and ras p21 expression in 90 cases of GTD and 30 normal placenta were determined by polymerase chain reaction - single strand conformation polymorphism analysis (PCR - SSCP) and streptavidin peroxidase conjugated method (SP). Results : All of the normal placenta were negative on the mutation in exon 1 of C - Ha - ras gene and p21 expression. The mutation rate in exon 1 of C - Ha - ras gene was 36.7 % in malignant GTD and 43.3 % in complete hydatidiform moles (CM) which culminated in GTD. Both of them were 16.7 % higher than that of the CM without GTD formation. The mutation rate of grade Ⅰ, Ⅱ and Ⅲ malignant GTDs were 15.4 % , 40 % and 71.4 % (P< 0.01) respectively. The positive rate of p21 varied in the different CMs, and was 83.3 % in the CM developing to GTD which was significantly higher than the 43.3 % of the CM without GTD (P< 0.005), and higher than 60 % of the malignant GTD (as well (P< 0.05). Conclusion : The development and progression of GTD is associated with the mutation of C - Ha - ras and expression of p21 protein. It may be considered as one of the diagnostic marker forecasting GTD undergoing malignant changes.
Key Words Gestational trophoblastic disease (GTD) c - Ha - ras gene mutation p21 protein

妊娠滋养细胞疾病(GTD)的良性病变有明显的侵袭性倾向和恶性转化潜能 ......