【摘要 】 目的 一些以血管平滑肌细胞(VSMC)增殖为特点的血管疾病，在病变部位常有巨噬细胞浸润。本研究巨噬细胞集落刺激因子(MCSF)在VSMC生长调节中的作用。方法 实验采用培养大鼠主动脉VSMC，细胞增殖观察指标采用氚标胸腺嘧啶核苷掺入法，并用Northern blot技术测定原癌基因表达。结果 (1)L929细胞上清液(富含MCSF)及重组MCSF以剂量依赖关系刺激氚标胸腺嘧啶核苷掺入；(2)VSMC在接受刺激后表达某些原癌基因，如c-fos、c-myc、erg-1和JunB；(3)凝血酶、PDGF、bFGF与MCSF在促增殖作用上具有协同作用。结论 MCSF与其它生长因子协同作用，通过自分泌/旁分泌机制调控VSMC增殖，从而可能在血管病变的形成和进展中起重要作用。

Macrophage colony-stimulating factor and vascular smooth muscle cell proliferation

Zhu Dingliang¹ , Gogusev J² , Marche P³ . 1 Department of Cellular & Molecular Biology, Shanghai Institute of Hypertension, Buijin Hospital, Shanghai Second Medical University, Shanghai 200025. 2 INSERM U90, Paris 75015, France. 3 CNRS URA 1482, Paris 75015, France.

【Abstract 】 Objective In several vascular diseases an abnormal vascular smooth cell(VSMC) proliferation is often associated with the presence of macrophages. we examinedwhether macrophage colony-stimulating factor (MCSF) might play a role in the control ofVSMC growth. Methods Culturedrat VSMC were obtained from thoracic aortae. Cell proliferation was evaluated by measuring [³ H]-thymidineincorporation and by determing proto-oncogene expression assessed with Northern blotanalysis. Results Ourresults showed that (1) The addition of L929 cell supernatant (a rich source of MCSF) orof recombinant MCSF promoted DNA synthesis in a concentration-dependent manner, an effectthat could be abrogated by the presence of a specific anti-MCSF antibody. (2) The additionof L929 supernatant induced of c-fos, c-myc, egr-1 and junB expression. (3) It was furtherdemonstrated that MCSF acted in synergy with thrombin, platelet-derived growth factor(PDGF) or basic fibroblast growth factor (bFGF) in promoting VSMC growth. Conclusion These data suggest that in concert with other growthfactors MCSF affects the growth of cultured VSMC through autocrine/paracrine mechanisms.These effects confer to MCSF an important role in the development of vascular lesionformation.

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