【摘要】 目的 探讨抗氧化维生素对高脂饮食兔动脉粥样硬化(AS)及DNA甲基化水平的影响。方法 用显微镜比较兔高脂饮食组，高脂抗氧化维生素组主动脉AS的程度并用高效液相色谱层析(HPLC)方法分析脾脏DNA的甲基化水平。结果 高脂抗氧化维生素组较高脂饮食组兔动脉平滑肌细胞及泡沫细胞的增生明显减少，AS明显减轻，而且脾脏DNA的甲基化水平明显增高。结论 抗氧化维生素有保护DNA甲基化的作用，并可能通过此作用而影响基因转录，减轻AS。可能是抗氧化维生素减轻AS的另一种因素。

    The antioxidative vitamins reduce atherosclerosis and protectDNA methylation of high-fat diet rabbits SongFujin, Chen Duanxia, Yang Di, et al. PUMC Hospital, CAMS and PUMC, Beijing 100037

    【Abstract】 Objective To study the effectof antioxidative vitamins on high-fat diet rabbits in atherosclerosis and DNA methylationlevel. Methods The microscope and the HPLC was usedto compare the atherosclerosis and the DNA methylation level in the high-fat diet groupand the antioxidative vitamins high-fat diet group of rabbit spleen. Results The methylation level was higher and the atherosclerosis level waslower in antioxidative vitamins high-fat diet group of rabbits than that in high-fat dietgroup. And also there was a much less hyperplasia of smooth muscle cells and foam cells inthe former group. Conclusion The results suggestedthat antioxidative vitamins may effect the gene transcription by protecting DNAmethylation, and may therefore reduce atherosclerosis. This may be another pathway ofantioxidative vitamin reducing atherosclerosis caused by high-fat diet.

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