【摘要】 目的 探讨丹参提取物Rxa对正常肝细胞(L02细胞)增殖速率的影响。方法 应用流式细胞术(FCM)分别测定L02细胞Rxa处理组(La组)和未处理组(L组)0、2、4、8、12小时G0-G1、S、G2-M期细胞指数，同步进行Ki-67、Cyclin D1、PCNA蛋白表达的免疫组化分析。结果 La组细胞增殖速率较快，Ki-67抗原表达相对较强，CyclinD1、PCNA表达相对提前。结论 Rxa可能促进L02细胞增殖。

Effects of Rxa on L02 cell proliferation and the expression of Ki-67, Cyclin D1, PCNA protein

LU Qiping, WU Zaide, WANG Wei, et al.

    Department of Surgery, Wuhan General Hospital of Guangzhou Command, Wuhan 430070

【Abstract】 Objective To investigate the effect of Rxa, a kind of extract of RSM, on the proliferation rate in cultured human hepatocytes (L02 cells). Methods L02 cells were divided into two groups: non-treatment group (L group) and Rxa-treated group (La group, Rxa 2 mmol/L). Indices of G0-G1 phase cells, S phase cells and G2-M phase cells were measured by using flow cytometry at 0, 2, 4, 8 and 12 h, respectively, and the expression of Ki-67, Cyclin D1, PCNA protein were simultaneously detected by using immunohistochemical method. Results Index of hepatocytes in each phase reached the peak more quickly in Rxa-treated group than in non-treatment group, and the Ki-67 expression was also higher than that one. Cyclin D1 and PCNA expressed earlier in the hepatocytes treated with Rxa (P<0.01), which expressed sooner than in the hepatocytes not treated with Rxa about 2～4 h. Conclusion Rxa may be play an important role in the hepatic proliferation, which may be contributed to the triggering expression of Ki-67, Cyclin D1 and PCNA, by which the cell cycle transport was speeded up.

    【Key words】 Proliferation Cell-cyclin Hepatocyte RSM

为探讨国药丹参对正常肝细胞是否具有促增殖作用 ......