摘要 目的 :研究微卫星DNAs的遗传不稳定性与慢性粒细胞白血病(CML)加速、急变的关系。方法 :采用基础PCR-银染方法对9例加速期和8例急变期CML患者的骨髓细胞与其慢性期标本位于染色体17p的Mfd41和18q的DCC两个微卫星序列进行比较分析。结果 :17例CML加速期或急变期患者中有8例出现微卫星不稳定性(MSI)或杂合性丢失(LOH),占总病例数的47.5%。6例(加速期2例,急变期4例)出现DCC改变,占总病例数的35.3%；2例(加速期和急变期各1例)出现Mfd41改变,占总病例数的11.8%。结论 :微卫星DNAs的遗传不稳定性可能参与CML加速或急变的演变。

Genetic instability ofmicrosatellites Mfd41 and DCC in the evolution of chronic myelogenous leukemia Li Ge, Song Yuhua, Qian Linsheng,et al. Institute of Hematology,CAMS and PUMC, Tianjin 300020

    Abstract Objective:To explore the relationship between the genetic instability of microsatellite and theevolution of chronic myelogenous leukemia(CML).Methods:The loss of heterozygosity(LOH) andthe microsatellite instability(MSI) of two polymorphic microsatellite markers, DCC andMfd41,which located on chromosome 18q and 17p respectively, were assayed by standard PCR-silver staining analysis in bone marrow cells from 17 CML patients progressing fromchronic phase to accelerated phase or blast crisis. Results: LOH or MSI of the twomicrosatellites were demonstrated in 8/17 (47.5%) patients in accelerated/blastic phases.For DCC, genetic instability was revealed in 2 of 9 patients in accelerated phase and in 4of 8 in blast crisis. For Mfd41, genetic instability was revealed in 1 of 9 patients inaccelerated and in 1 of 8 in blast crisis. Conclusion: Genetic instability of DCC andMfd41 may play a role in the evolution of CML.

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