邵咏红 黄英伟 陈劲松 崔金环 刘晓松 刘玉韶 陈小雨 邵咏红 黄英伟 528000 广东省佛山市第一人民医院肾内科；陈劲松 刘玉韶 陈小雨 内分泌科；崔金环 刘晓松 临床研究所 中华肾脏病杂志 1999 0 15 1


    关键词：血管紧张素转换酶；基因多态性；非胰岛素依赖型糖尿病；糖尿病肾病 期刊 zhszbzz 0 论著 fur -->


    

【摘要 】 目的 探讨血管紧张素转换酶(ACE)基因插入/缺失(I/D)多态性与Ⅱ型糖尿病(NIDDM)及其肾脏合并症发病的关系。方法 应用聚合酶链反应(PCR)扩增技术检测了109例NIDDM患者及155例健康对照者的ACE基因I/D多态性。结果 位于ACE基因第16内含子的I/D多态性经PCR技术扩增后分为三种基因型：纯合子缺失型(DD)，纯合子插入型(II)及杂合子插入/缺失型(ID)。109例NIDDM患者与155例正常对照组之间基因型及等位基因频率差异均无显著意义；NIDDM合并肾病者(DN)的基因型与未合并肾病者无显著性差异，但等位基因则有显著性差异(D、I等位基因为0.45和0.55对0.30和0.70)(P<0.02)；NIDDM病程≤1年即伴有肾病者与病程≥5年仍无肾病者比较，DD型及D等位基因均显著高于无肾病组(P均<0.05)，后者以II型及I等位基因占绝对优势。结论 ACE基因多态性与NIDDM发病无关，而与其肾脏合并症则明显相关，DD型是DN的易感基因，而II型则为其保护基因。

Insertion/deletion polymorphism in the angiotensin converting enzyme gene is not associated with NIDDM,but a predictor for progression of diabetic nephropathy in a Chinese population

SHAO Yonghong,HUANG Yingwei,CHEN Jingsong,et al. Foshan First Hospital,Guangdong Foshan 528000

【Abstract 】 Objective To investigate the relationship between insertion/deletion (I/D) polymorphism of ACE gene and NIDDM and its renal complication. Methods Polymerase chain reaction (PCR) was used to determine the genotypes for an I/D polymorphism in intron 16 of the ACE gene in 109 cases of NIDDM and 155 healthy subjects as controls. Results The I/D polymorphism in intron 16 of the ACE gene was categorized into three genotypes:two deletion alleles (genotype DD),heterozygous alleles (genotype ID),and two insertion alleles (genotype II).The genotype pattern of DD,ID,II and the D allele were similar between 109 cases of NIDDM and 155 control subjects (20% ......