关键词：细菌L型；医院感染；质粒谱；同源性分析；多重耐药性
摘要 目的 为了全面防止医院感染，我们对细菌L型的医院感染进行了研究。方法 对医院内环境中800份标本及医院感染患者918份标本分离出的金黄色葡萄球菌L型、大肠埃希氏菌L型及铜绿假单胞菌L型进行了质粒谱分析和药敏试验。结果 本院环境及医院感染患者细菌L型总检出率分别为19.0%和16.7%。不同来源的3种细菌L型的多重耐药性无显著性差异。不同来源3种细菌L型的同源性比例均较高，分别为90.3%、75.0%、84.2%。结论 细菌L型医院感染患者的病原菌主要来自医院内环境。
中国图书资料分类法分类号 R378
Studyon the L-form Bacteria Nosocomial Infection with
Plasmid Profile Analysis
Chen Duan Shan Bin Chen Dechang Zhang Qiuxia Pian Hu aiyan
Department of Clinical Laboratory, First Hospital,
Kunming Medical college, Kunming 650032
Abstract OBJECTIVE In order to prevent nos ocomial infection, the L-form bacteria were studied. METHODS 800 samples carried out from the hospital envionment and 918 samples from the nosocomial infected patients. Three strains of L-forms (Staphy oeocaus aureus L-form, Eschherichia colo L-from, Pandomonos L-form). were iso lated and identified. RESULTS The general c ulture positive rates of 3 strains of L-form bacteria of the hospital environme nt and the nosocomial patients were 19.0% and 16.7% respectiveoy. The multiple a ntbiotic resistance tests of the three strains from two different origins were o f no significante difference (P>0.01). The homology ratios of the three strains of L-form bacteria from two different orig ins were ruther high ie, 90.3%, 75.0% and 84.2% respectively. CONCLUSIONS The pathogenic organisms of the nosocomial infe cted patients by L-form bacteria may be contamincded from the hospital environm ent.
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