目的 探讨小儿原发性肾病综合征(NS)脂质代谢紊乱机制。方法 测定了68例小儿NS及120例健康对照组7项血脂指标，采用聚合酶链反应-单链构象多态性(PCR-SSCP)技术检测其载脂蛋白E(ApoE)基因多态性。并采用SDS-PAGE检测NS组尿蛋白类型。结果 所有7项指标在携不同ApoE等位基因(Apoε₂ 、Apoε₃ 及Apoε₄ )的患儿之间均无显著性差异(P>0.05)，而携Apoε₃ 等位基因型的选择性蛋白尿(SPU)组血总胆固醇(TC)、甘油三酯(TG)显著高于同型的非选择性蛋白尿组(NSPU)(P<0.05)。NSPU组的Apoε₂ 等位基因频率显著高于健康儿童组(12.96% vs 5.00%，P<0.05)。结论 小儿原发性NS的脂质紊乱主要与其蛋白代谢异常有关而与ApoE基因多态性无明显关系。但携Apoε₂ 等位基因的NSPU患儿有进行性肾脏损害的可能。

Objective To investigate the molecular mechanism of abnormal lipid in children with idiopathic nephrotic syndrome (INS). Methods 7 lipoprotein metabolism parameters including serum TC, TG, HDL-C, LDL-C, ApoA-I, ApoB and LP(a) of 68 children wiht INS as well as 120 age and sex-matched healthy children were determined. Single-strand conformational polymorphism (PCR-SSCP) were used to determine the ApoE genotypes of the patients and the types of urine protein were determined by urine protein sodium dodecylsulphate polyacrylamide. Results All 7 parameters had no difference among the cases with Apo ε₂ , ε₃ and ε₄ allele genotypes (P>0.05) ......