王东旭 房殿春 周晓东 刘为纹 300142 天津，解放军第254医院(王东旭)；；第三军医大学西南医院(房殿春、周晓东、刘为纹) 中华消化杂志 1999 0 19 2


    关键词：胃肿瘤；APC/MCC基因；微卫星不稳定性；杂合缺失 期刊 zhxhzz 0 论著 fur -->


    

【摘要】 目的 探讨5q微卫星不稳定性(MSI)与APC/MCC基因杂合缺失(LOH)的关系。方法 应用PCR-SSLP及PCR-RFLP技术分析52例手术切除胃癌组织中MSI及APC/MMC基因LOH。结果 5q MSI检出率为34.0%(16/47),APC/MCC基因LOH率为31.4%(11/35)。早期胃癌5q MSI阳性率为66.7%(2/3),APC/MCC LOH率为50%(1/2)；进展期分别为31.8(14/44)，30.3%(10/33)。两组间无显著差别(P>0.05)。MSI及杂合缺失与肿瘤大小、浸润深度、淋巴结转移及临床分期无关。粘液(印戒)细胞癌APC/MCC LOH率(55.6%)显著高于高、中分化管状腺癌(P<0.05)。胃、肠两型胃癌5q MSI及APC/MCC LOH差异无显著性及5q MSI与APC/MCC LOH无相关性(P>0.05)。结论 染色体5q MSI有APC/MCC基因LOH在两型胃癌的早期发生及发展中起一定作用。染色体5q可能是胃癌的易感部位。

Microsatellite instability of chromosome 5q and loss of heterozygosity at APC/MCC genetic loci in gastric carcinoma

WANG Dongxu, FANG Dianchun, ZHOU Xiaodong, et al.

    Department of Gastroenterology, PLA 254 Hospital, Tianjin 300142

【 Abstract】 Objective To evaluate the role of microsatellite instability (MSI) of chromosome 5q and loss of heterzygosity(LOH) at APC/MCC gene in the development and progeression of gastric carcinoma(GC).Methods MSI of 5q and LOH of APC/MCC were investigated in 52 surgically resected specimens of GC using PCR-SSLP and PCR-RFLP analysis. Results Positive MSI was found in 16 (34.0%) of 47 successfully amplified cases and LOH of APC/MCC was detected in 11(31.4%) of 35 cancer speciments. The frequencies of MSI and LOH in early stages of GC were 66.7%(2/3) and 50.0%(1/2), respectively, while that in advanced stage of GC, they were 31.8(14/44) and 30.3%(10/33). There was no significant difference between were early and advanced stages MSI and LOH were not correlated to tumor size、 invasion depth、 lymph node metastasis and clinical staging, LOH in mucoid (Signet ring) cell group(55.6%) was higher than that of well or moderately differentiated group (0.0%)(P< 0.05). There was no significant difference of LOH and MSI between intestinal and gastric type of GC and MSI of 5q was not correlated to LOH of APC/MCC gene(P> 0.05). Conclusions MSI of 5q and LOH of APC/MCC play imporatent roles in the carcinogensis and progression of both intestinal and gastric types of GC and chromosome 5q may be a susceptible target site of GC.

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