马晋平 詹文华 蔡世荣 彭俊生 汪建平 510080 广州，中山医科大学第一医院胃肠胰腺外科 中华消化杂志 1999 0 19 4


    关键词：胃肿瘤；端粒酶；端粒重复扩增法 期刊 zhxhzz 0 论著 fur -->


    

【摘要】 目的 探讨胃癌组织中端粒酶活性的临床意义，研究非放射性端粒重复扩增法(TRAP)检测端粒酶的可行性。方法 用TRAP银染法检测端粒酶在58例胃癌，12例胃腺瘤和9例胃溃疡组织的表达情况。结果 84.5%(49/58)的胃癌组织中检测到端粒酶活性，正常粘膜对照组无1例检测到端粒酶活性(P<0.001)，12例胃腺瘤、9例胃溃疡组织中各有1例检测到端粒酶活性。胃癌组织端粒酶的表达与年龄、肿瘤大小、分化程度、浸润深度、淋巴结转移及TNM分期等临床病理参数无明显相关(P>0.05)。结论 端粒酶是一种较理想的胃癌肿瘤标志物；TRAP银染法是一种值得推广的检测端粒酶活性的方法。

Study of telomerase activity in gastric carcinomas

MA Jinping, ZHAN Wenhua, CAI Shirong, et al.

    Devision of gastrointestinal and pancreatic surgery, First Affiliated Hospital, SUN Yatsen University of Medical Sciences, Guangzhou 510080

【 Abstract 】 Objective To appraise the telomerase activity in human gastric carcinomas and the clinical significance and to evaluate the feasibility of non-radioisotopic TRAP assay to detect telomerase activity. Methods Telomerase activity was detected by a non-isotopic PCR-based TRAP assay (Telomeric repeat amplification protocol) in tissue samples from 58 gastric carcinomas, and their matched normal tissues, 12 gastric adenomas and 9 gastric ulcer. Results None of the normal tissues exhibited telomerase activity. In contrast, 49 of 58 gastric cancer specimens were positive for telomerase activeity with a 84.5% positive rate (P < 0.001) ......

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