【摘要】 目的 克隆中国人肥胖基因，并进行序列分析和在大肠杆菌中表达。方法 采用RT-PCR法从中国人脂肪组织RNA中扩增出肥胖基因(Obesegene)，进行核苷酸顺序分析，并将该基因克隆入原核表达载体，在大肠杆菌中表达。结果 克隆了中国人肥胖基因，其cDNA顺序与已报道的白种人的序列完全一致，并成功在大肠杆菌中获得表达。结论 本项工作为进一步研究肥胖基因在肥胖症和糖尿病等病人中的表达情况及探讨肥胖基因的作用机制打下了基础。

    The molecular cloning,sequence analysis and high efficiency expression in E.coli of Chinese obese gene hou Wei,Miao Weimin,Zhou Bingrong,et al.Department ofMicrobiology,Second Military Medical University,Shanghai,200433

    【Abstract】 Objective To probe into thepositional cloning of the Chinese obese gene, thereby providing an important approach toresearch of the mechanisms of obesity. Methods The obese gene wasamplified from the Chinese adipose tissue RNA by RT-PCR. Results Sequencinganalysis showed that the Chinese obese gene cDNA sequence was identical to that ofCaucasian reported previously. The gene was cloned into a prokaryotic expression vectorand high efficiency expression was realized in E.coli. Conclusion Thiswork is the basis for further researches on mechanisms of the obese gene product-leptinand its potential effect on treatment of obesity and diabetes mellitus, etc.

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