吴永贵 林善锬 周江华 200040 上海医科大学附属华山医院肾内科 中华内分泌代谢杂志 1999 0 15 2


    关键词：血管紧张素转换酶抑制剂；糖尿病；肾皮质；胰岛素受体；大鼠 期刊 zhnfmdxzz 0 论著 fur -->


    

【摘要 】 目的 研究血管紧张素转换酶抑制剂(ACEI)对糖尿病大鼠肾皮质细胞膜胰岛素受体表达的调节。方法 实验大鼠随机分三组：单侧肾切除组(C组，6例)；糖尿病组(D组，7例)及糖尿病苯那普利治疗组(DB组，7例)。观察四周后各组血糖、血胰岛素及血肌酐水平和体重、肾重及肾重/体重之比的变化，测定血浆、肾皮质及髓质血管紧张素转换酶(ACE)活性，采用Western杂交分析肾皮质细胞膜胰岛素受体蛋白表达。结果 苯那普利治疗四周后能够改善糖尿病大鼠血糖、血胰岛素及血肌酐水平的异常，对糖尿病肾脏肥大有显著抑制作用，对血浆、肾皮质及髓质ACE活性抑制分别达92.00%，88.77%与73.40%。肾皮质细胞膜胰岛素受体蛋白表达比对照组增加约2.10倍，苯那普利治疗四周后对此有明显的下调作用。结论 苯那普利可下调糖尿病状态下肾皮质细胞膜胰岛素受体蛋白高表达，这可能是其对糖尿病肾脏保护作用的重要机制之一。

Regulation of renal cortex cellmembranes insulin receptor in diabetic rats by ACEI

WU Yonggui,LIN Shanyan,ZHOU Jianghua.Division of Nephrology,Hua Shan Hospital,Shanghai Medical Univsersity,Shanghai,200040

【Abstract 】 Objective Toinvestigate into the regulation of renal cortex cell membranes insulin receptor indiabetic rats by angiotensin converting enzyme inhibitor(ACEI). Methods Therats were randomly divided into following groups: uninephrectomized rats (group C, n=6), streptozotocin-induced diabetic rats (group D, n=7) and diabetic rats treated withbenazepril (an ACEI), (group DB, n=7). Blood glucose, serum insulin, serum creatininelevel as well as body weight, kidney weight and kidney weight/body weight were observedafter 4 weeks of study. ACE activities in plasma, renal cortex and medulla were measuredby fluorimetric assay. The expression of renal cortex cell membranes insulin receptor weredetermined by Western blot analysis. Results After 4 weeks oftreatment, benazepril could ameliorate hyperglycemia, decrease serum insulin and increaseserum creatinine level. It could also significantly supress kidney hypertrophy in diabeticstate. ACE activities in plasma, renal cortex and medulla were reduced by approximately92.00%, 88.77% and 73.40%, respectively. Western blot analysis showed that the expressionof renal cortex cell membranes insulin receptor was increased by about 2.10 folds indiabetic rats, however, benazepril might significantly down-regulate its expression. Conclusion Benazeprilmay down-regulate the increased expression of renal cortex cell membranes insulin receptorprotein in diabetic rats, which may be one of the important mechanism of its nephroprotection.

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