杨忠广 许纯孝 马庆铮 杨忠广 250012 济南 山东医科大学附属医院泌尿外科；许纯孝 马庆铮 山东省立医院泌尿外科 中华泌尿外科杂志 1999 0 20 10


    关键词：膀胱肿瘤；癌；灌注法 期刊 zhmnwkzz 0 论著 fur -->


    

摘要 目的 探讨体外膀胱灌注噻替哌和丝裂霉素C药物浓度和作用时间对膀胱癌细胞杀伤作用的影响。 方法 用不同浓度的抗肿瘤药物与人膀胱移行细胞癌细胞系BIU-87共同培养至一定时间，采用MTT比色法测定细胞存活率。 结果 细胞存活率与二药的药物浓度和作用时间均呈明显的线性相关(P<0.01)，而且主要与浓度有关；当超过一定作用时间后，存活率仅与药物浓度呈负相关(P<0.01)。 结论 只有保证有效的作用时间，尽量增加药物剂量和有效浓度，才能显著提高膀胱腔内化疗效果，减少复发。

A pharmacodynamic study ofthiotepa and mitomycin C on human bladder cancer cell line in vitro

YANG Zhongguang,XU Chunxiao,MA Qingzheng.

    Department of Urology,The Affiliated Hospital of Shandong Medical University,Jinan 250012

Abstract Objective Tostudy the cytotoxic effect of thiotepa (THT) and mitomycin C (MMC) on human bladder cancercell line in vitro. Methods Human bladder transitional cell carcinoma cell line BIU-87was cultured for varying period with varying concentrations of the antineoplastic agentsadded.MTT colorimetric assay was used to determine the cell survival rate. Results Drugconcentration and exposure period were significantly correlated with the cell survivalrate (P<0.01),drug concentration being the predominant.When the effective exposureperiod for the drugs was ensured and accessed the cell survival rate was only negativelycorrelated with drug concentration (P<0.01). Conclusions When the effective exposureperiod is guarranted,increase of drug dose and the effective concentration wouldsignificantly enhance the therapeutic effect of the intravesical chemotherapy,thusreducing the recuurence rate of bladder cancer after surgical management.

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