何斌 金锡御 杨唐俊 金欢胜 曾毅 刘素英 400037 重庆， 第三军医大学第二附属医院泌尿外科(何斌、杨唐俊、金欢胜、曾毅、刘素英)，第一附属医院泌尿外科(金锡御) 中华泌尿外科杂志 1999 0 20 7


    关键词：药物耐受性；转基因 期刊 zhmnwkzz 0 论著 fur -->


    

【摘要】 目的 建立多药耐药细胞系。 方法 将带有mdr1 cDNA全序列的逆转录病毒载体pHaMDR通过脂质体直接导入到敏感的人膀胱癌细胞系BIU-87细胞中。 结果 经60～100ng/ml秋水仙素筛选21天后，克隆出高强度MDR表型的耐药细胞系，基因组DNA PCR扩增表明mdr1 cDNA已掺入到BIU-87细胞基因组中。 结论 应用转基因方法可建立多药耐药细胞系，该细胞系具有耐药强度高，耐药性稳定等特点。

Construction of a multidrug resistant cell line with gene transfer

HE Bin, JIN Xiyu,YANG Tangjun,et al.

    Department of Urology, the Second Affiliated Hospital of The Third Military Medical University, Chongqing 400037

【Abstruct】 Objective To construct a multidrug resistant cell line. Methods The retroviral plasmid pHaMDR that contains the full sequences of mdr1 cDNA has been transfected into BIU-87 cell line, a sensitive bladder cancer cell line, by liposome. Results A highly resistant cell line was astablished after cultured with selective medium containing 60～100ng/ml colchicine for 21 days. The PCR results confirmed that mdr1 cDNA had been transduced into the genome of the sensitive bladder cell line. Conclusions Gene transfer is a sound method to construct multidrug resistant cell line. The transfected cell line showed high degree and stability of multidrug resistance.

     ......

您现在查看是摘要页，全文长 9579 字符。