【摘要】 目的 检测老年人膀胱移行细胞癌组织中P16、P53、bc1-2及c-erbB-2的基因表达，探讨其与膀胱癌病理分级、临床分期和转移的关系。 方法 应用免疫组化SP法检测55例老年人膀胱癌组织中P16、P53、bc1-2及c-erbB-2的基因表达。 结果 55例膀胱癌中P16、P53、bc1-2及c-erbB-2的阳性率分别为43.6%、49.1%、41.8%及41.8%，多种癌基因激活和抑癌基因失活与肿瘤病理分级、临床分期和有无转移有密切的关系。70.9%的肿瘤有上述癌基因和(或)抑癌基因的异常表达，其中50.9%的肿瘤同时有多个基因的表达异常。 结论 肿瘤的多基因分析比单基因分析更有价值，癌基因bc1-2、c-erbB-2和抑癌基因P16、P53的表达异常及协同作用可能在老年人膀胱癌的发生、发展中起重要作用。

P16, P53, bc1-2 and c-erbB-2 geneexpression in senile patients with transitional cell carcinoma of urinary bladder

CHEN Ting, LI Jing, CAO Hui, et al. Departmentof Microbiology, Jining Medical College, Shandong 272113

【Abstract】 Objective To study theexpression of P16, P53, bc1-2 and c-erbB-2 protein in senile patients with transitionalcell carcinoma of urinary bladder and the correlation between the tumor grade, clinicalstage and metastasis. Methods Immunohistochemical method was used todetect the P16, P53, bc1-2 and c-erbB-2 gene expression in 55 senile cases withtransitional cell carcinoma of urinary bladder. Results Positiverates of P16, P53, bc1-2 and c-erbB-2 in 55 cases of bladder cancer were 43.6% ......