【摘要】 目的 探讨老年吸烟者肺癌H-ras基因变化的意义。 方法 对33例吸烟肺癌和22例不吸烟肺癌患者采用纤维支气管镜毛刷刷取支气管脱落细胞，应用PCR-RFLP方法进行H-ras基因突变检测。 结果 肺癌中H-ras基因总突变率为52.7%(29/55)，其中吸烟组肺癌H-ras基因突变率为66.7%，不吸烟组肺癌为31.8%，二者差异有显著性(P<0.05)，吸烟组肺鳞癌患者H-ras基因突变率为70.0%，明显高于不吸烟组肺鳞癌患者的16.7%(P<0.05)。 结论 提示H-ras基因可能是被烟草激活的一个特殊位点，吸烟是导致H-ras基因突变的重要因素。

Detection of H-ras oncogene mutationin elderly smokers with lung cancers

YING Kejing, LIU Fuguang, ZHANG Xing, et al. TheSecond Affiliated Hospital, Zhejiang University, Hangzhou 310009

【Abstract】 Objective To study therelationship between the H-ras oncogene and lung cancers in elderly smokers. Methods Thesamples were collected from 55 consecutive patients with tissue diagnosed lung cancers byfiberoptic bronchoscopic brushing. Mutation in coden 12 of H-ras oncogene was detected inthe exfoliated cells using PCR-RFLP method (polymerase chain reaction-restriction fragmentlength polymorphisms). Results H-ras oncogene mutation was present in52.7%(29/55)of total patients with 70.0%(23/33) of smokers and 31.8%(7/22) of nonsmokers(P<0.01).Subgroup analysis showed that H-ras oncogene mutation was present in75.0%(15/20) of smokers with squamous cell lung cancer as compared to only 16.7%(1/6) ofnonsmokers with squamous cell lung cancer (P>0.05). Conclusions Ourresults suggest that coden 12 of the H-ras oncogene may be a specific target for themutagenic activation by tobacco smoke, and smoking is an important factor in the inductionof H-ras oncogene mutation.

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