孙利炜 焦立新 王承训 郑丽芬 李永富 130061 长春市儿童医院 中华实验和临床病毒学杂志 1999 0 13 4


    关键词：婴儿；呼吸道合胞病毒；基因扩增；DNA 期刊 zhsyhlcbdxzz 0 论著 fur -->


    

【摘要】 目的 探讨婴幼儿呼吸道合胞病毒(RSV)感染的分子流行病学情况。方法 采用随机扩增多态DNA(RAPD)技术，对长春市儿童医院1992～1994年分离并鉴定的96株RSV和一株标准RSV进行RAPD分析。结果 所有RSV毒株都有扩增带，共有四种带型。不同疾病来源的RSV基因型不同。来源毛细支气管炎的RSV有87.14%为R₁ 型。结论 RAPD技术能从分子水平了解婴幼儿RSV感染情况；R₁ 型RSV可能为引起婴幼儿毛细支气管炎的主要病原。

Molecular epidemiology of respiratory syncytial virus infection in infants

SUN Liwei, JIAO Lixin, WANG Chengxun, et al.

    Changchun State Hospital of Pediatrics, ChangChun 130061

【Abstract】 Objective To search for the molecular epidemiology of respiratory syncytial virus (RSV) infection in infants.Methods The randomly amplified polymorphism DNA (RAPD) technology was used to study 96 RSV strains isolated and identified from the patients in Changchun State Hospital of Pediatrics during 1992-1994 and 1 refrerence strain of M type.Results All strains were typable, four RAPD patterns were observed, different diseases have different types. 87.14% of the patients′ RSV strains had the same RAPD pattern(R₁ ).Conclusion RAPD method is a rapid, easy and high resolution technique, with which we can know on the molecular level the microbial infection state. There was epidemic of R₁ type infection during the period surveyed.

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